β-Glucuronidase in lymphatic tissues

Phenoxyacetohydrazide Schiff bases: β-glucuronidase inhibitors.

Phenoxyacetohydrazide Schiff base analogs 1-28 have been synthesized and their in vitro β-glucouoronidase inhibition potential studied. Compounds 1 (IC50=9.20±0.32 µM), 5 (IC50=9.47±0.16 µM), 7 (IC50=14.7±0.19 µM), 8 (IC50=15.4±1.56 µM), 11 (IC50=19.6±0.62 µM), 12 (IC50=30.7±1.49 µM), 15 (IC50=12.0±0.16 µM), 21 (IC50=13.7±0.40 µM) and 22 (IC50=22.0±0.14 µM) showed promising β-glucuronidase inhi...

Continuous Spectrophotometric Assay for β - Glucuronidase

A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...

Histochemical localization of ß-glucuronidase in some vertebrate tissues

ß-Glucuronidase is the novel enzyme known to present in the tissues of several vertebrates. It can be detected by biochemical, histochemical and immunocytochemical techniques in bacteria, plant and animal. The traditional method used for histochemical demonstration of ß-glucuronidase is quite tedious and substrate utilized in a given concentration is not affordable to researchers of the develop...

Distribution of lymphatic tissues in duck caeca.

A β-glucuronidase (GUS) based cell death assay.

We have developed a novel transient plant expression system that simultaneously expresses the reporter gene, β-glucuronidase (GUS), with putative positive or negative regulators of cell death. In this system, N. benthamiana leaves are co-infiltrated with a 35S driven expression cassette containing the gene to be analyzed, and the GUS vector pCAMBIA 2301 using Agrobacterium strain LBA4404 as a v...